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Bifunctional protein for improved stent performance and endothelial regeneration
Author(s) -
Baumer Yvonne,
Leder Christoph,
Schoenberger Tanja,
Ziegler Melanie,
Filzmayer Christina,
Buehring HansJoerg,
Gawaz Mainrad,
Schlosshauer Burkhard
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.644.8
Subject(s) - progenitor cell , homing (biology) , restenosis , stem cell , extracellular matrix , microbiology and biotechnology , endothelial stem cell , bifunctional , cd34 , chemistry , endothelial progenitor cell , stent , medicine , biology , biochemistry , in vitro , ecology , catalysis
Restenosis and thrombosis are undesired effects after stent implantation. Reduced or missing re‐endothelialization of injured coronary arteries shall be abolished by improving stem cell homing at the side of injury to support their differentiation into intact endothelium. We developed a bifunctional protein binding to collagen at the vessel lesion site and to stem/progenitor cells. ELISA studies demonstrated that this bifunctional protein binds to collagen type1 comparably strong as the control protein. Employing stem cell marker overexpressing cells and human CD34 + endothelial progenitor cells on a multi substrate array (MSA) spotted with 14 different extracellular matrix proteins demonstrated the bifunctionality and specificity of the protein. This specificity was substantiated in flow chamber adhesion experiments. Furthermore, we tested colony forming ability and by this endothelial like differentiation of CD34 + ‐cells on fusion protein coated surfaces. In conclusion, this bifunctional protein is a promising construct to support re‐endothelialization and therefore reducing restenosis and thrombosis risk after stent implantation. Supported by the German Ministry of Education and Research (BMBF) # 01GU0727 and 01GU0726.