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Maternal expression of atrial natriuretic peptide and the fetal programming of cardiac hypertrophy
Author(s) -
Armstrong David WJ,
Tse M Yat,
Wong Philip G,
Pang Stephen C.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.644.2
Subject(s) - atrial natriuretic peptide , medicine , offspring , endocrinology , muscle hypertrophy , homeostasis , biology , pregnancy , genetics
The natriuretic peptide system (NPS) is a key regulator of cardiovascular homeostasis. Mice lacking atrial natriuretic peptide (ANP) display chronic hypertension with salt‐sensitivity and cardiac hypertrophy (CH). The effect of maternal ANP expression on the development of CH in offspring is unknown. Objective To determine the effect of maternal ANP genotype on the development of CH in ANP heterozygous (ANP +/− ) offspring born to either ANP wild‐type (+/+) mothers (ANP +/−WT offspring) or ANP knockout (−/−) mothers (ANP +/−KO offspring). Methods 9‐week old male ANP +/−WT and ANP +/−KO mice were treated with normal salt (NS; 0.8% NaCl) or high salt (HS; 8.0% NaCl) diet for 5 weeks. Mice were euthanized, organs were weighed, and left ventricular (LV) gene expression was measured using real‐time qPCR. Results LV‐to‐body weight (LV/BW) ratio did not differ between ANP +/−WT and ANP +/−KO fed NS. HS increased LV/BW ratio in ANP +/−WT but not in ANP +/−KO . Gene expression of corin, iNOS, ACE, AT 1a , sGC‐α1 and ‐β1 were increased, and NPR‐C was decreased in ANP +/−WT , and unaltered in ANP +/−KO . Conclusions Maternal ANP ablation appears to protect against the development of CH in the ANP +/− mouse. Compensatory changes in the ANP −/− mother during development may confer salt‐resistance in the ANP +/− mouse later in life, the mechanism of which remains to be elucidated. (Support: Heart & Stroke Foundation of Ontario) Grant Funding Source : Heart and Stroke Foundation of Ontario