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D‐Glucose Attenuation of 1‐Methyl‐4‐Phenylpyridinium ion (MPP+) Toxicity in C6 Glial Cells
Author(s) -
Ramesh Badisa,
DarlingReed Selina,
Soliman Karam F
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.641.2
Subject(s) - glutathione , toxicity , chemistry , programmed cell death , substantia nigra , mitochondrion , membrane potential , biochemistry , apoptosis , biology , endocrinology , dopaminergic , dopamine , enzyme , organic chemistry
Impaired mitochondrial function in the substantia nigra is one of the most likely cause of Parkinson's disease. In the present study, we investigated the protective effects of glucose against MPP+ toxicity. Results obtained indicate that MPP+ caused significant cell death in 2 mM glucose, while 10 mM glucose showed significant protection against MPP+ effects. Morphological observations revealed that MPP+ treated cells in 2 mM glucose significantly changed cell shape and size, while in 10 mM glucose; cells appeared similar to the control. MPP+ in 2 mM glucose decreased significant mitochondrial respiration, membrane potential and glutathione levels in a dose dependent manner while 10 mM glucose significantly restored them. Moreover, MPP+ in 2 mM glucose arrested the cells at G0/G1 and G2/M phases, demonstrating its dual inhibitory nature. However, in 10 mM glucose, MPP+ caused G0/G1 arrest only. The protective effects of glucose were not observed with cocaine, demonstrating that this protection is compound specific. In summary, the results obtained indicate that the loss of cell viability in 2 mM glucose group with MPP+ treatment was primarily related with significant decrease in mitochondrial respiration, membrane potential, total glutathione levels, and dual arrest of cell phases, while 10 mM glucose rescues glial cells from MPP+ induced toxicity by restoring these factors.(Supported by NIH grant RR 03020)