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Lifelong sedentary behavior aggravates detrimental age associated effects on bone structure of C57BL/6 mice
Author(s) -
Fonseca Helder Martins,
Gonçalves Daniel Moreira,
Figueiredo Pedro Carvalho,
Mota Paula Gonçalves,
Duarte José Alberto
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.637.3
Subject(s) - osteocyte , trabecular bone , femur , ageing , wheel running , medicine , endocrinology , bone remodeling , physical activity , chemistry , osteoporosis , surgery , osteoblast , physical therapy , biochemistry , in vitro
The aim of this study was to compare the femoral bone structure of lifelong sedentary vs active C57BL/6 mice. Thirty male C57BL/6 mice, aged 2 months, were either: i) sacrificed after arrival (young group; Y; n=10); ii) kept in cages equipped with running wheels, where they could perform voluntary running (aged active group; AA; n=10); iii) kept in cages without running wheel (aged sedentary group; AS; n=10). After 23 months of differential housing AS and AA animals were also sacrificed. In all animals the right femur was collected and processed for histomorphometryc analysis of both epiphyses and mid diaphysis. Right hind limb muscles were collected for citrate synthase (CS) activity determination to confirm physically active and sedentary behaviors. Differences in trabecular microarchitecture and cortical bone geometry were identified between AA and AS groups. AA mice showed significantly increased trabecular perimeter (Tb.Pm) and trabecular number (Tb.N) and decreased trabecular separation (Tb.Sp) at both epiphyses when compared to AS mice. Differences in osteocyte density (Oc.N/mm 2 ) were identified between AS and Y mice but not between AA and Y mice. Our results evidence that skeletal changes usually indentified as a result of ageing, are at least in some extent, due to sedentary behavior. Maintenance of a physically active lifestyle is therefore essential for delaying changes in bone quality throughout life. Grant Funding Source : FCT grant SFRH/BD/38110/2007