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Immunohistochemical localisation of VEGF in Cynops pyrrhogaster teeth development
Author(s) -
MIWA Yoko,
Sunohara Masataka,
SATO Iwao,
SHIMADA Kazuyuki
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.634.2
Subject(s) - ameloblast , odontoblast , vascular endothelial growth factor , enamel organ , microbiology and biotechnology , enamel paint , immunohistochemistry , matrix (chemical analysis) , chemistry , anatomy , biology , medicine , vegf receptors , pathology , dentin , dentistry , chromatography
Japanese newts, Cynops pyrrhogaster has a polyphyodont dentition that enables us to observe its tooth development thoroughly at a time with sequential appearance of teeth. Vascular endothelial growth factor (VEGF) is a key regulator of blood vessel endothelium. We examined immunohistochemical localization of VEGF and its receptors, and showed the specific expression pattern of VEGF and VEGF receptor in Cynops pyrrhogaster tooth from “dentine matrix formation and mineralixation stage” to “enameloid maturation and enamel‐like matrix formation stage”. The intensity of the immunoreactivity to VEGF and its receptor VEGF receptor‐2 (VEGFR‐2) were positive in inner enamel epithelium and odontoblasts at “dentine matrix formation and mineralixation stage”. We postulate that the different expression of VEGF in inner enamel epithelium, ameloblast and odontoblast during each stage of tooth development may affect a tooth germ formation.