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The effects of aging on muscle loss and NF‐κB levels of various tissues in rats
Author(s) -
LaGuire Tiev C.,
Kohlen Corinne R.,
Reaves Scott K.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.621.2
Subject(s) - medicine , endocrinology , skeletal muscle , anabolism , soleus muscle , sarcopenia , gastrocnemius muscle , western blot , kidney , biology , lung , catabolism , metabolism , biochemistry , gene
The objective of the study was to examine the influence of aging on food intake, tissue and organ mass and the levels of NF‐κB in various tissues were examined. The transcription factor, Nuclear Factor Kappa‐B (NF‐κB), is associated with both catabolic and anabolic pathways of muscle metabolism and may be involved in age‐related muscle loss. Adult male Sprague‐Dawley rats, aged 6 (Adult) and 21 months (Old) were fed a diet for 10–17 days. Old rats consumed less grams of food per body weight (BW) each day than Adult rats (1.45% g diet/g BW vs. 2.4% g diet/g BW) although when intake data were expressed as g/diet per day there was no significant difference between groups. For skeletal muscle tissue, the average mass of gastrocnemius and soleus (g muscle/g BW) was significantly lower in Old rats but there was no difference for tibialis anterior. Levels of NF‐κB (p65 or RelA) were measured by Western blot analysis in gastrocnemius, tibialis anterior, quadriceps, soleus, lung, heart, kidney and liver. NF‐κB levels were higher in gastrocnemius of Old rats as compared to Adult rats. There were no significant age‐specific differences in NF‐κB levels in any other tissue examined. Interestingly, the level of NF‐κB was highest in lung in both age groups as compared to any other tissue. Data from this study suggest that aging affects muscle loss and NF‐κB in a tissue‐specific manner.