Premium
Caloric Restriction Attenuates Many Age‐Related Changes in Skeletal Muscle Mitochondrial Physiology
Author(s) -
Lanza Ian R,
Li Eileena,
Jakaitis Daniel,
Ali Bushra,
Nair K. Sreekumaran
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.621.1
Subject(s) - skeletal muscle , mitochondrion , chemistry , medicine , endocrinology , andrology , biology , biochemistry
Mitochondrial function declines with age but increases with caloric restriction (CR). Mitochondria and muscle fibers were prepared from young (8–11mo) and old (22–24mo) AL‐fed and old CR mice. State 3 respiration was lower in OAL vs. YAL, but preserved in OCR ( table). Normalizing to protein content eliminated the age‐effect in AL mice but amplified the effect of CR, indicating that CR enhances the intrinsic mito function. ROS production in isolated fibers increased with age in AL but not CR mice ( table). Coupling was reduced in OAL but not OCR. These results indicate that mito capacity declines with age in situ and in vitro, apparently driven largely by decreased mitochondrial protein content. CR increases mito capacity and efficiency independently of mito protein content.YAL OAL OCRFibers (pmol/s/m g tiss) CI 54.0±4.1 38.9±1.6 * 50.8±3.0 † CII 44.4±3.4 36.9±1.4 * 45.0±2.3 † CI+II 63.4±5.5 51.8±2.5 * 67.0±3.4 †Mito (pmol/s/m g tiss) CI 38.9±1.8 21.5±2.6 * 30.7±2.0 † CII 30.6±0.9 19.6±1.2 * 24.7±1.4 † CI+II 59.0±1.6 34.0±1.9 * 46.7±2.7 †Mito (pmol/s/m g prot) CI 8350±635 7071±1082 10422±863 § CII 6536±414 6309±506 8408±676 § CI+II 12601±762 11063±1156 15949±1381 §H 2 O 2 (pM/s/mg tiss) CI 10.0±1.8 30.8±3.7 * 15.0±2.0 † CI+II 141.4±20.9 187.2±53.8 101.1±13.6 †P:O State IV to III 2.94±0.03 2.71±0.02 * 2.92±0.05 †Means±SEM. * P<0.05 OAL vs YAL, † P<0.05 OCR vs OAL, § P<0.05 OCR vs. OAL & YAL.