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Regulation of Annexin A1 in the macula densa: association with neuronal nitric oxide synthase and cyclooxygenase‐2
Author(s) -
Paliege Alexander,
Seidel Saskia,
Roschel Tom,
Mutig Kerim,
Bachmann Sebastian
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.620.2
Subject(s) - nos1 , macula densa , nitric oxide synthase , cyclooxygenase , nitric oxide , furosemide , endocrinology , chemistry , medicine , enzyme , biology , biochemistry , renin–angiotensin system , blood pressure
Aim of the present study was to determine the effects of salt transport on the expression of Annexin A1 (ANXA1) in the macula densa (MD) and its association with cyclooxygenase‐2 (COX‐2) and neuronal nitric oxide synthase (NOS1). The MD associated expression of ANXA1, NOS1 and COX‐2 were determined by immunohistochemistry and cell counting on kidney sections of furosemide treated rats. Association of ANXA1 with NOS1 and COX‐2 was studied by double labelling immunofluorescence. Furosemide treatment caused a strong increase in the number of ANXA1 expressing macula densa cells (51±16 vs. 126±19 cells/100 glomeruli; p<.05). The number of NOS1 and COX‐2 expressing cells were similarly increased (NOS1: 84±14 vs. 186±14 cells/100 glomeruli; p<.05 and COX‐2: 54±9 vs. 183±24 cells/100 glomeruli; p<.05). Double labelling immunofluorescence showed a frequent (90–100%) co‐expression of ANXA1 with NOS1 and COX‐2 in control‐ and furosemide treated animals. In summary we have shown that ANXA1 expression in the MD of rats is stimulated by furosemide treatment and colocalizes with the MD enzymes NOS1 and COX‐2. ANXA1 may therefore be critically involved in the regulation of MD signalling.