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Ventilatory effect following 10 days concomitant exposure to neonatal intermittent hypoxia (NIH) and neonatal caffeine treatment (NCT) in 12‐days old rats
Author(s) -
Julien Cecile,
Joseph Vincent,
Bairam Aida
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.613.5
Subject(s) - caffeine , medicine , apnea , hypoxia (environmental) , apnea of prematurity , hypoxic ventilatory response , plethysmograph , intermittent hypoxia , ventilation (architecture) , anesthesia , respiratory system , obstructive sleep apnea , biology , chemistry , gestational age , pregnancy , oxygen , mechanical engineering , organic chemistry , engineering , genetics
Caffeine is regularly used to alleviate apneas in newborn infants. Its efficiency is thought to be related to increased respiratory response to hypoxia. However, we still lack knowledge on interactions between chronic NCT and NIH. Rat pups were exposed to NIH (nadir 5%O2, 6 cycles/h followed by 1h normoxia, 24h/day from P3‐12) and daily gavaged with caffeine (15 mg/kg; NIH+NCT) or water (NIH+NWT). Two other groups exposed to normoxia but gavaged with caffeine (NCT) or water (NWT) were used. At end of exposure, ventilation (VE ml/100g/min) was measured under baseline and in hypoxia (12% O2, 20 min) by plethysmography. Apnea frequency was assessed under baseline (>2 missed breaths). Baseline VE was: 194±18; 223±11*, 234±22* and 192±14 for NWT, NCT, NIH+NCT and NIH+NWT, respectively; *p0.05 vs NWT. Apnea frequency was significantly higher in NIH+NWT pups and lower in both NCT and NIH+NCT. The increase in VE was correlated with the decrease in apnea frequency (p=0.04). In response to hypoxia, the % increase from baseline was +2% for NCT; −10% for NIH+NWT; and +18% for NIH+NCT (p=ns). In conclusion: Chronic exposure to NCT in presence of NIH enhances baseline ventilation but not the hypoxic response. This suggests that caffeine by improving baseline VE acts to decrease apnea frequency. (CIHR, FRMI)