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Effects of exercise training on cardiac mitochondrial apoptosis in ovariectomized rats
Author(s) -
Hsu ChihChao,
Ou HsiuChung,
Lee ShinDa
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.601.5
Subject(s) - ovariectomized rat , apoptosis , medicine , cytochrome c , tunel assay , endocrinology , h&e stain , caspase 3 , mitochondrion , immunohistochemistry , programmed cell death , biology , estrogen , microbiology and biotechnology , biochemistry
Cardiac apoptosis is found in ovariectomized rats; however, the effects of exercise training on cardiac apoptosis is still unclear in menopausal or bilateral oophorectomied women was available. The purpose of this study was to evaluate the therapeutic effects of exercise training on cardiac mitochondrial apoptotic pathways. Methods Eight sham‐operated rats and eight ovariectomized rats at 5–6 months of age were served as negative (sham) and positive (OVX) control. Additional 8 ovariectomized rats underwent treadmill running exercise 1 hour daily for 3 month. After exercise training or sedentary status, the excised hearts were measured by positive TUNEL assays, Hematoxylin‐eosin staining and Western Blotting. Results Exercise training significantly prevented the increasing of whole heart weight and OVX‐induced cardiac cell apoptosis. In addition, exercise training decrease the OVX‐induced tBid, Bad, Bax, Bak, cytochrome c overexpression, and caspase‐9 and caspase‐3 activation. Conclusions Exercise training suppressed ovariectomy‐induced cardiac apoptosis through inhibiting mitochondrial apoptotic pathways in rat models. The findings may provide one of possible therapeutic approaches for preventing cardiac apoptosis in menopausal or bilateral oophorectomied women.