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Biochemical alterations in coronary endothelial luminal membranes during ischemia and reperfusion in isolated perfused guinea pig hearts
Author(s) -
Knabb Maureen T,
PérezAguilar Sandra,
TorresTirado David,
RamiroDíaz Juan,
Rubio Rafael
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.598.4
Subject(s) - ischemia , glycocalyx , guinea pig , contractility , chemistry , endothelium , perfusion , membrane , medicine , biology , pharmacology , biochemistry
Ischemia/reperfusion has marked effects on the thickness of the coronary endothelial glycocalyx but little is known about the biochemical alterations associated with this structural change. Lectinic and glycan‐containing molecules have been implicated in flow‐induced changes in myocardial conduction and contractility but the mechanism is unknown. In order to identify the effects of ischemia and reperfusion on endothelial flow‐specific biomarkers, isolated perfused guinea pig hearts were treated with ischemia followed by reperfusion. Luminal coronary endothelial membranes were isolated via the silica pellicle method in control, ischemic, or ischemia/reperfusion treated hearts. Flow sensitive proteins including PECAM, VCAM, ENaC, B 2 , AT 1 and AT 2 receptors were decreased in luminal membranes in reperfused as compared to ischemic hearts. Anionic glycoconjugates (GAGs) quantified with Alcian blue on PVDF membrane decreased from 0.95 to 0.35 mg GAG/mg protein in ischemic hearts but showed partial recovery with reperfusion to 0.52 mg GAG/mg protein. These results indicate that ischemia leads to loss of glycosylated proteins that are partially restored with reperfusion. However, flow sensitive proteins are lost from the endothelium, which may explain the deleterious effects of reperfusion following ischemia. Funded by CONACyT SEP‐42567, CONACyT‐SALUD 2004‐C01‐156, Fulbright‐ Garcia Robles #9583.