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Quantification of inflammatory and Toll‐like receptor gene expression in insulin resistant horses
Author(s) -
Stokes Ashley Michelle,
Carlisle Katherine,
Madara Elise,
McGeachy Matthew,
Lavie Nicholas,
Keowen Michael,
Garza Frank,
Andrews Frank
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.597.8
Subject(s) - insulin resistance , tlr2 , inflammation , insulin receptor , insulin , biology , receptor , tlr4 , endocrinology , horse , gene expression , pi3k/akt/mtor pathway , irs2 , toll like receptor , medicine , signal transduction , gene , immunology , microbiology and biotechnology , genetics , innate immune system , paleontology
The objectives of this study were to quantify the expression of several inflammatory‐related genes from the Toll‐like receptor signaling pathway found in leukocyte samples taken from insulin resistant horses in comparison to normal horses. Genes of interest are Toll‐like receptors 2 and 4, (TLR2, 4), Cluster of Differentiation Factor 14 (CD‐14), Interleukin‐8 (IL‐8), Phosphoinositide 3‐kinase (PI3K), and nuclear factor NFKBIA inhibitor IkBa. Alterations should reflect chronic low‐grade inflammation resulting from insulin resistance as found in other species. Objectives were to identify horses with insulin resistance and quantify expression of these genes in insulin resistant horses in comparison to normal horses. Insulin resistance was determined in 21 horses using an IV glucose‐insulin tolerance test. Total leukocyte RNA was extracted from a single baseline jugular venous sample from normal (n=13) and insulin resistant (n=8) adult horses. TaqMan RT‐PCR was used to examine the samples and quantify the genes of interest. Significance was determined after calculating ΔΔCT values with a P‐value considered significant < 0.05. There was significant down‐regulation of inflammatory inhibitors (IkBa, PI3K) and up‐regulation of indicators of TLR involvement and inflammation (TLR‐4, IL‐8) indicating the possibility of the existence of chronic inflammation in insulin resistant horses.