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Vitamin C reduces AT 1 R and AT 2 R activation in the rostral ventrolateral medulla of hypertensive sucrose‐fed rats
Author(s) -
Oliveira Thaís Galvão Cesar,
OliveiraSales Elizabeth Barbosa,
Casarini Dulce Elena,
Bergamaschi Cássia Toledo,
Heimann Joel C,
Campos Ruy Ribeiro,
Dolnikoff Miriam Sterman
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.594.8
Subject(s) - endocrinology , medicine , tbars , rostral ventrolateral medulla , chemistry , vitamin c , blood pressure , heart rate , lipid peroxidation , oxidative stress
The aim of this study was to evaluate blood pressure (BP), renal sympathetic nerve activity (rSNA), protein expression and activation of AT 1 R and AT 2 R in the RVLM of control (C, n=22), sucrose‐fed (20% sucrose solution, 30 days; SC, n=24) and sucrose‐fed rats treated with vitamin C (150 mg/Kg/day, last 7 days; SCvC, n=22). Thiobarbituric acid reactive substance (TBARS) was measured in serum. Insulin, Ang II and glucose were measured in plasma. Protein expression was evaluated by western blot and the receptors’ activation was evaluated by ELISA in tissue slices. BP, rSNA, insulin, Ang II and TBARS levels increased (P<0.05) in SC compared to C. In SCvC, Ang II and insulin levels were similar to SC although, BP, rSNA and TBARS were diminished. AT 1 R protein expression in the RVLM was similar in all groups while there was a 74% increase (P<0.05) in AT 2 R protein expression in SC compared to C. In relation to C, an intense (P<0.05) AT 1 R (116%) and AT 2 R activation (417%) were found in SC. In SCvC, AT 2 R protein expression levels were 43% lower than that of SC and were similar to those of C. AT 1 R and AT 2 R activation were also 39% and 29% lower in SCvC compared to SC. These results suggest that AT 1 R and AT 2 R activation in RVLM is involved in rSNA and hypertension in sucrose‐fed rats. Supported by FAPESP.