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Resveratrol protects the renal microcirculation during sepsis
Author(s) -
Holthoff Joseph H,
Mayeux Philip R
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.593.1
Subject(s) - sepsis , kidney , medicine , acute kidney injury , perfusion , microcirculation , renal blood flow , resveratrol , pharmacology
Over 200,000 patients die annually from sepsis in the US. In severe cases, acute kidney injury (AKI) develops and increases mortality to near 70%. Sepsis induced by cecal‐ligation and puncture (CLP) in the mouse leads to renal microvascular dysfunction and the generation of reactive nitrogen species (RNS) in the capillary microenvironment of kidney. The polyphenol resveratrol (Rsv) has been shown to have potent anti‐oxidant properties. Thus, we propose that Rsv might protect the kidney against injury during sepsis by serving as an RNS scavenger. Rsv (30 mg/kg) was given to mice 6 h prior to CLP, at CLP, and again 6 h post‐CLP. At 18 h, CLP reduced renal capillary perfusion from 323 ± 14 μm/sec in sham to 222 ± 18 μm/sec in CLP (P<.05) and capillary volumetric flow from 11.8 ± 0.3 pl/sec in sham to 8.7 ± 0.5 pl/sec in CLP (P<.05) in the cortex as measured by intravital video microscopy. Rsv treatment prevented the decline in both renal perfusion and volumetric flow. Kidneys of septic mice also showed an increase in oxidation of dihydrorhodamine 123 compared to sham animals, indicating an increase in RNS generation. Rsv also blunted this increase. Collectively, these data indicate that Rsv can preserve the renal microvascular circulation and reduce RNS generation in the kidney during sepsis. Thus, Rsv or agents like it may have clinical application as a novel treatment to prevent sepsis‐induced renal injury. Supported by NIH DK075991.