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BPP‐10c isolated from Bothrops jararaca venom has antithrombotic effect in rats
Author(s) -
Ianzer Danielle Alves,
Xavier Carlos H,
FragaSilva Rodrigo A,
Faria Fernanda,
Camargo Antonio C M,
Santos Robson A S,
Teixeira Mauro M
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.589.7
Subject(s) - partial thromboplastin time , antithrombotic , medicine , bleeding time , bothrops jararaca , thrombin time , prothrombin time , venom , fibrinogen , thrombus , pharmacology , anesthesia , coagulation , chemistry , snake venom , platelet , biochemistry , platelet aggregation
Bradykinin potentiating peptides (BPPs) were the first ACE inhibitors described. Recently, we identified new BPPs from B.jararaca venom and showed that some of them, including BPP‐10c, were able to reduce arterial pressure of SHR without affecting ACE activity. Male wistar (WT) and spontaneously hypertensive rats (SHR) were used to evaluate tail bleeding time after acute or chronic administration of vehicle (NaCl 0.9%) or BPP‐10c (71nmol/Kg) (n=7 each). We also assessed venous thrombus weight from vena cava after acute iv injections of vehicle or BPP‐10c (n=4). Both treatments with BPP‐10c increased bleeding time in WT (28.0±1.8 vs. 11.7±1.6; 27.3±2.3 vs. 16.8±3.2min; P<0.05, respectively) and SHR (15.3±1.6 vs. 5.8±0.9; 20.4±3.0 vs. 9.6±2.0min; P<0.05, respectively). BPP‐10c also reduced the thrombus weight of WT (−29%) and SHR (−48%) compared to control groups (7.6±0.9 vs. 10.7±0.8; 6.2±1.0 vs. 11.8±0.6mg; P <0.05, respectively). Prothrombin time, activated partial thromboplastin time and fibrinogen levels were not changed. In conclusion, BPP‐10c has antithrombotic effect and represents a potential pharmacological tool to recover homeostasis and treat both hypertension and coagulation diseases. Mechanisms involved require further investigation. Support: FAPESP and CNPq.