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Antioxidant and anti‐inflammatory properties of apo A‐I milano
Author(s) -
Purushothaman Meerarani,
Puru Priya,
Cimmino Giovanni,
Badiom Juan J
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.589.18
Subject(s) - oxidative stress , apolipoprotein b , chemistry , antioxidant , inflammation , proinflammatory cytokine , cholesterol , microbiology and biotechnology , endocrinology , medicine , biochemistry , biology
The apolipoprotein A‐I Milano (ApoA‐I Milano), the mutant form of ApoA‐I was found to reduce plaque size and composition in an experimental model and enhanced reverse cholesterol transport evident primarily in HDL. We hypothesize that ApoA1 Milano may also possess antioxidant and anti‐inflammatory properties and prevent atherosclerotic disease process. The mouse macrophage cell line J774A‐1 was incubated with apoA‐I Milano or HDL and exposed to oxLDL or TNF‐alpha. The oxidative stress was quantified by the dichlorodihydrofluorescein method, the DNA binding activity of NF‐kB was measured by ELISA and the interleukin‐6 gene expression by PCR. The oxidative stress induced by oxLDL was markedly reduced by apoA‐I Milano or HDL incubation. Similarly the increase in NF‐kB activity induced by oxLDL or TNF‐alpha was reduced by apoA‐I Milano and HDL. However, the induction of inflammatory cytokine IL‐6 gene by TNF‐alpha was markedly downregulated by apoA‐I Milano (P<0.0001) than by HDL (P<0.003). These results indicate apoA1 Milano may possess antioxidant and anti‐inflammatory properties. Our data suggest that apoA‐I Milano downregulates the oxidative and inflammatory response associated with atherosclerotic disease process. Furthermore this study indicates that apoA‐I Milano exhibited enhanced anti‐inflammatory mechanism compared to ApoA1/HDL.