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ICAM‐1 is Key Molecule in Carbamylated LDL‐induced Monocyte Adhesion
Author(s) -
Apostolov Eugene,
Ray Debarti,
Savenka Alena,
Shah Sudhir,
Basnakian Alexei
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.589.17
Subject(s) - monocyte , cell adhesion molecule , adhesion , icam 1 , in vivo , chemistry , endothelium , apolipoprotein b , vcam 1 , cell adhesion , knockout mouse , microbiology and biotechnology , immunology , medicine , biochemistry , biology , receptor , cholesterol , organic chemistry
Carbamylated LDL (cLDL) is a strong proatherogenic factor that is associated with chronic renal failure (CRF). It causes endothelial toxicity and exacerbates monocyte adhesion to endothelial cells. Monocyte adhesion is a key atherogenic process that leads to extensive housing of macrophages and formation of foam cells filled with lipids, the major marker and driving mechanism of atherosclerotic plaque formation. Recently, we showed that ICAM‐1 was the most employed molecule for cLDL‐mediated monocyte in vitro. Here we explored whether ICAM‐1 mediates the cLDL‐induced adhesion to vascular endothelium in in vivo model. For this we utilized the animal CRF model and urea‐consuming (UC) model in Apolipoprotein E knockout (KO) mice. Our results suggest that cLDL is elevated in sera and aortic walls of CRF and UC mice as compared to control groups. ICAM‐1 expression and number of housed macrophages in aortic wall and atherosclerotic plaques are significantly increased in experimental animals. Finally, monocyte cLDL‐induced adhesion was studied in ICAM‐1 KO mice. Our data suggest that cLDL induces significantly higher monocyte adhesion in wild type mice as compared to ICAM‐1 KO. Taken together, our data suggest that ICAM‐1 is a leading molecule that mediates mechanism of cLDL‐induced monocyte adhesion. The study was supported by AHA grant to E.A. and NIH/NHLBI R21 to A.B.