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Modulation of Response to Stress by Estradiol Benzoate and Selective Estrogen Receptor Agonists
Author(s) -
Sabban Esther Louise,
Harris Heather A,
Serova Lidia I
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.588.1
Subject(s) - endocrinology , estradiol benzoate , medicine , locus coeruleus , corticosterone , catecholaminergic , estrogen , chemistry , estrogen receptor , receptor , gene expression , biology , hormone , ovariectomized rat , gene , central nervous system , catecholamine , biochemistry , cancer , breast cancer
Pretreatment with estradiol benzoate (EB) was found to modulate response of HPA axis and gene expression in several catecholaminergic neuronal locations in OVX female rats exposed to single immobilization (1xIMO) stress. Here we examined ER subtypes involved using selective agonists for ERα (PPT) or ERβ (WAY‐200070) on response to 1xIMO and repeated (6xIMO) stress. OVX female rats received 21 daily injections of either EB (25 μg/kg), PPT (10 μg/kg), WAY‐200070 (10 μg/kg) or vehicle. EB and PPT, but not WAY‐200070, reduced body weight by about 20% and uterine weight 8‐fold Both EB and PPT increased plasma corticosterone about 2–3 fold, but prevented any further rise with either 1xIMO or 6xIMO, indicating an ERα mediated mechanism. In the locus coeruleus (LC), the rise in DBH mRNA with both stress paradigms was abrogated with EB or PPT, while, WAY‐200070 blocked the response of DBH mRNA to 1xIMO but not 6xIMO. In the nucleus of solitary tract (NTS), the rise in TH and DBH mRNAs with 1xIMO or 6xIMO was absent, or greatly attenuated in EB or PPT treated animals. In most cases, WAY‐200070 inhibited the response to single but not repeated IMO. The results demonstrate that pretreatment with estradiol, or ER selective agonists, modulate the stress triggered induction of gene expression of NE biosynthetic enzymes in LC and NTS, with ER selectivity depending on the duration of the stress. (NIH grant NS 28869 by Wyeth Research)