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Effect of L‐carnitine intake on tolerance to physical exercise, oxidative stress and vascular reactivity in obese trained rats
Author(s) -
Priviero Fernanda B. M.,
RojasMoscoso Julio,
GómezCampos Rossana,
Silva Carmem Valgas,
Carneiro Everardo M,
Antunes Edson,
Zanesco Angelina
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.570.1
Subject(s) - endocrinology , sodium nitroprusside , medicine , lipid peroxidation , phenylephrine , oxidative stress , chemistry , nitric oxide , blood pressure
We assessed the effect of L‐Car ingestion on tolerance to exercise (EX) and vascular reactivity of rats fed with a hyperlipidic diet (HD) and submitted to EX. Male Wistar rats were divided in sedentary (SD), exercised (EX), SD + L‐Car (SDLC) and EX + L‐Car (EXLC). After 4 weeks of HD intake, rats underwent to supplementation and an EX program of running for further 4 weeks. Tolerance to exercise was tested until exhaustion. Concentration‐response curves for acetylcholine (ACh), sodium nitroprusside (SNP) and phenylephrine (PE) were obtained in mesenteric artery (RMA). Plasma MDA and SOD levels were measured. Body weight gain was reduced in SDLC (Δ: 137 ± 4 g) compared to SD (Δ: 188 ± 10 g). No further reduction was found in EXLC. Tolerance to exercise was higher in EX (15 ± 1 min) compared to SD (7 ± 1 min). L‐Car raised tolerance to exercise in EXLC (22 ± 2 min) and SDLC (12 ± 1 min). In RMA, L‐Car increased the potency (pEC 50 ) of ACh in EXLC (7.89 ± 0.03), but not in SDLC (7.67 ± 0.06), compared to SD (7.65 ± 0.04). Responses to SNP and PE were not altered. L‐Car decreased MDA levels in SDLC (2.4 ± 0.2 μM) and EXLC (2.9 ± 0.4 μM) compared to SD (4.6 ± 0.2 μM). SOD activity was not changed. In conclusion, L‐Car improved body weight loss and tolerance to exercise in SD. Together with EX, L‐Car improved endothelium‐dependent relaxation in RMA probably through decreasing lipid peroxidation. Financial Support: FAPESP