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MiRNA Expression Patterns during Skeletal Muscle Regeneration
Author(s) -
Chen Yongxin,
Gelfond Jonathan,
McManus Linda M.,
Shireman Paula K.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.568.5
Subject(s) - cardiotoxin , regeneration (biology) , microrna , skeletal muscle , biology , progenitor cell , microbiology and biotechnology , myogenesis , myocyte , cell growth , stem cell , andrology , anatomy , medicine , genetics , gene
MiRNAs regulate many biological processes. Expression of miRNAs during mouse muscle (tibialis anterior) regeneration was analyzed by qPCR‐array at baseline and days 1, 3, 4, 7, 21 post‐cardiotoxin injury. Based on histology, satellite cell proliferation occurred at days 1–4 after injury and myoblast differentiation began at day 3, with overlap of both events at days 3–4. Over 100 miRNAs changed >2‐fold ( p <0.01) after injury compared to baseline.miRNA Fold Change Day 1 3 4 7 21miR‐1 −104 −94 −28 −4.6 −1.6 miR‐133a −32 −127 −34 −5.2 −1.7 miR‐682 7.4 5.2 1.5 2.8 1.8Expression of muscle‐specific miR‐1 and −133a significantly decreased immediately after injury and returned to baseline, a pattern consistent with a role in maintaining muscle differentiation. Conversely, another subset of miRNAs, including miR‐682, significantly increased at early days after injury, suggesting regulatory roles in satellite cell proliferation. In addition, some miRNAs were up‐regulated in both early and late days after injury. Interestingly, the expression time course of the above 3 miRNAs was remarkably similar during in vivo muscle regeneration and in vitro myogenic progenitor cell proliferation and differentiation. These studies define miRNA expression patterns that may regulate muscle repair; manipulation of specific miRNAs may improve muscle regeneration. Supported by the Veterans Administration HL07446 and HL074236.