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Pro‐apoptotic effects of retinoic acid on activated canine neutrophils
Author(s) -
Channabasappa Shankaramurthy,
Stewart Sarah,
Caldwell Sarah,
Carr Anthony,
Singh Baljit
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.565.3
Subject(s) - retinoic acid , tunel assay , apoptosis , innate immune system , lipopolysaccharide , inflammation , terminal deoxynucleotidyl transferase , annexin , neutrophil extracellular traps , chemistry , immunology , biology , microbiology and biotechnology , immune system , biochemistry , gene
Neutrophils are the key cellular component of innate immune system. Accumulation of activated neutrophils that live longer cause considerable tissue injury, which is central to the pathogenesis of many inflammatory conditions. Retinoids are a class of lipophilic compounds that include natural vitamin A and its synthetic derivatives which have anti‐inflammatory effects. We examined the effect of retinoic acid on apoptosis in normal and activated neutrophils using light microscopy, electron microscopy, terminal deoxynucleotidyl transferase dUTP nick end labelling (TUNEL) assay, and Annexin V staining. Our results indicated that treatment with 1 μg/ml bacterial lipopolysaccharide (LPS) for 12 hours delayed the spontaneous neutrophil apoptosis as compared to untreated cells. But treatment of cells with both LPS (1 μg/ml) and retinoic acid (1 and 5 μM) abolished inhibitory effects of LPS on neutrophil apoptosis in a concentration‐ dependant manner. These results provide new insights on the effects of retinoic acid on neutrophil apoptosis and this effect could enhance the resolution of inflammation. Grant Funding Source : Research supported by WCVM Vitamin Class Action Settlement Fund