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Mouse Genotype Alters Palatal Fusion Response to Nicotine
Author(s) -
Serrano Maria J.,
Svoboda Kathy K.H.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.565.11
Subject(s) - nicotine , embryo , in vivo , biology , andrology , anatomy , medicine , microbiology and biotechnology , genetics , neuroscience
OBJECTIVE Our previous work demonstrated that nicotine inhibited palatal fusion in vitro in a dose dependent manner. We asked if the mouse genotype would affect palatal fusion when exposed to nicotine. The objective of this study is focused on understanding the risk of maternal smoking as a factor for facial cleft defects. METHODS Palatal shelves from mouse strains (CD1, ND4, C57and 129S) at age E13.5 were cultured in serum free media (control), and nicotine (0.6 mM and 6 mM). Palates were harvested at 24 hours, 48 hrs and 72 hrs. Tissue was fixed in 4%PFA for paraffin embedding and H&E sections. In vivo study was done using pumps with nicotine in the four different mouse strains. Embryos mice were harvested at E15.5. Palatal tissue was harvested and fixed in 4% PFA for paraffin embedding and H&E sections. RESULTS The different mouse strains responded to the nicotine in different degrees. All palates exposed to nicotine failed to fuse. CD1 and ND4 strains were consistent in the palatal fusion pattern within litters. C57 and 129S had variable palate fusion. Some of the embryos had fused palates at embryonic day 13.5, while other embryos had undeveloped palatal shelves. The organ cultured palates from CD1 and ND4 mice treated with 0.6mM nicotine fused, while palatal shelves from C57 and 129S didn't fuse. The results in the in vivo experiment showed that the patterns in fusion occurred differently anterior to posterior especially in CD1 mice, the shelves were not fused in the posterior region. CONCLUSION The genetic background of mice influenced nicotine responses in palatal tissues. Grant Funding Source : March of Dimes