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Blueberries reduce lipid peroxidation and boost antioxidant enzymes in apoE knockout mice
Author(s) -
Wu Xianli,
Kang Jie,
Xie Chenghui,
Ferguson Matthew E.,
Badger Thomas M,
Nagarajan Shanmugam
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.564.20
Subject(s) - lipid peroxidation , antioxidant , catalase , biochemistry , chemistry , superoxide dismutase , glutathione reductase , apolipoprotein e , enzyme , medicine , endocrinology , biology , glutathione peroxidase , disease
ApoE knockout (ApoE −/− ) mice fed AIN‐93G diet (CD) formulated to contain 1% freeze‐dried whole wild blueberries (CD1% BB) were found to have significantly less atherosclerotic lesions in aorta. Biomarkers of lipid peroxidation, including F 2 ‐isoprostanes, hydroxyoctadecadienoic acids (HODEs) and hydroxyeicosatetraenoic acids (HETEs) were measured in serum. 8‐HETE, 12‐HETE, and 13‐HODE were found to be significantly lower in serum of CD1%BB fed animals. Gene expressions of seventeen major antioxidant/oxidant enzymes in aorta were further measured by PCR array. In pooled samples, three antioxidant enzymes, superoxide dismutase 2, glutathione reductase, thioredoxin reductase 1 were found to be up‐regulated and one antioxidant enzyme catalase was found to be down‐regulated in CD1%BB fed animals. These results were further verified by Real‐Time PCR in individual samples. Antioxidant activities of BB, especially the ability to inhibit lipid peroxidation and increase certain antioxidant enzymes may partly contribute to the protective effects of BB against atherosclerosis. USDA‐CRIS‐6251‐5100002‐06S .