Premium
Anti‐inflammatory, anti‐oxidant, anti‐microbial properties of human milk derived peptides
Author(s) -
Friel James Kenneth,
Tsopmo Apollo
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.556.7
Subject(s) - chemistry , peptide , tryptophan , viability assay , antioxidant , mtt assay , biochemistry , microbiology and biotechnology , cell growth , cell , amino acid , biology
Human milk (HM) digested and derived peptides (100 ug/mL) and tryptophan (Trp 50 ug/mL) were evaluated using MQ cells for NO assay, cell proliferation and cytotoxicity; cell viability and cytokines in MQ and Caco 2 cells and antioxidant properties with ORAC using standard protocols.Peptides ORAC Values (X±SD) TNFα (pg/ml) IL‐6 (pg/ml) NO (μM)Medium ‐ 781 976 35 Medium+LPS ‐ 6156 1136 90 23‐1 114±10 7684* 114* 60 23‐2 ‐ 5059 1068 150* 23‐3 ‐ 3952 1085 50 23‐4 3940±623 4188 1885 80 23‐5 3462±560 5259 1098 60 23‐6 3380±592 5590 1127 120* 23‐7 8205±552* 4933 1121 45 23‐8 6372±354* 4407 1055 55 Trp 50μg/ml 23563±317* 4869 1137 70Trp showed the highest ORAC value. Peptide 23‐7 (YGYTGA) and peptide 23‐8 (ISELGW) with a single Trp gave higher ORAC values. All peptides excluding peptide 23‐1 significantly inhibited the production of TNFα in LPS stimulated mouse macrophage cells. The profile of IL‐1 β was similar to that of TNF α. Peptides 23‐2 and 23‐6 enhanced the production of NO. Peptides 23‐7 and peptide 23‐8 produced relatively low quantities of vasodilator molecule NO. These peptides appear to be anti‐oxidant and anti‐inflammatory due to their unique structure. The MTT assay showed all peptides did not affect the viability of mouse macrophage cells but induced a moderate degree of growth proliferation in Caco 2 cells. 23‐8 (ISELGW) also exhibited strong anti‐microbial properties. This specific peptide may be suitable as additives to infant formulas to reduce inflammation and oxidation in vulnerable infants. Supported by AFMNet and MICH.