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Sodium selenate inhibits adipogenesis in vitro
Author(s) -
Wiacek Julie Lynn,
Kim KeeHong
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.547.8
Subject(s) - adipogenesis , sodium selenate , selenate , chemistry , selenium , viability assay , medicine , endocrinology , in vitro , microbiology and biotechnology , biology , biochemistry , organic chemistry
Adipogenesis refers to the differentiation of predadipocytes into mature adipocytes. This process is a potential target to treat or prevent obesity. Adipogenesis not only increases fat accumulation, but also elevates inflammation and oxidative stress. Selenium, an essential micromineral, has been shown to have potent anti‐oxidative and anti‐inflammatory properties. We therefore hypothesized that selenium can serve as a potential anti‐adipogenic agent. Sodium selenate was chosen over sodium selenite due to its relatively low toxicity in 3T3‐L1 cells. We found that selenate significantly inhibits adipogenesis in vitro in a dose dependent manner. Complete inhibition was observed at 50 microM, at which there were no negative effects on cell viability. We revealed that selenate's inhibitory action is limited to the early stage (day 0 to 2) of adipogenesis. Furthermore, we found that selenate reduced mRNA expression levels of adipogenic transcription factors, such as, peroxisome proliferator‐activated receptor‐gamma (PPAR‐gamma). More importantly, selenate was likely to maintain preadipocyte factor‐1 (Pref‐1), a preadipocyte marker gene expression, during adipogenesis. Taken together, our results support selenate's anti‐adipogenic properties in vitro . These effects are likely due to selenate‐mediated alteration of the adipogenic transcriptional program in the early stage of differentiation. This project is partially funded by the USDA‐AFRI grant and The Showalter Trust Fund Award. Grant Funding Source : USDA‐AFRI and The Showalter Trust Fund Award