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Type 2 Diabetes‐associated changes in the plasma lipidome in obese women
Author(s) -
Grapov Dmitry,
Adams Sean H,
Garvey W. Timothy,
Lok Kerry H,
Newman John W
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.542.1
Subject(s) - nefa , medicine , endocrinology , type 2 diabetes , diabetes mellitus , lipidomics , lipidome , arachidonic acid , chemistry , insulin , lipid metabolism , biochemistry , enzyme
Type 2 diabetes (T2D) is associated with elevated plasma fasting glucose, glycosylated hemoglobin (HbA1c), non‐esterified fatty acids (NEFA) and endocannabinoids (EC). An expanded assessment of lipidomic changes in diabetes is hypothesized to provide novel insights into metabolic changes associated with this malady. Targeted profiling of plasma lipids was performed in 43 diabetic and 12 non‐diabetic obese women. Plasma ECs (n=35), oxylipids (OxL; n=80), and NEFA (n=54) concentrations were measured. Multivariate statistics and regression analyses were used to mine the data matrix. Multivariate models showed a diverse array of NEFA, ECs, EC‐like compounds and OxLs to be significantly altered in the diabetic state. As opposed to the independent variables, an algorithm using subject age, BMI, HbA1c and fasting glucose was normally distributed and also segregated diabetic and non‐diabetic subjects. A linear model using measured lipids was developed that predicts the discriminating metric (adjR 2 =0.82) driven by OxL and EC subcomponents. Finally, this model showed that diabetic subjects had shifts in plasma levels of P450‐mediated arachidonic acid metabolites known to influence tissue plasminogen activator expression, which may parallel known shifts in vascular homeostasis. This work was supported by USDA‐ARS Project 5306‐51530‐016‐00D, NIH‐NIDDK R01DK078328‐01 and T32‐GM08799.