Premium
Cranberry phytochemicals act as non‐microbial antigens for different types of human peripheral blood mononuclear cells
Author(s) -
Rowe Cheryl A.,
Montero Cindy,
Nantz Meri P.,
Khoo Christina,
Percival Susan S.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.541.6
Subject(s) - peripheral blood mononuclear cell , proanthocyanidin , flow cytometry , immune system , chemistry , polyphenol , cell growth , cell , biology , biochemistry , in vitro , microbiology and biotechnology , immunology , antioxidant
To determine whether specific families of phytochemicals are able to interact with human peripheral blood mononuclear cell (PBMC) receptors and initiate an immune response, isolated human PBMC were incubated with three different extracts of cranberries representing anthocyanins, proanthocyanidins, or polyphenols. Proliferation of cells upon ex vivo stimulation and surface marker expression were measured by flow cytometry. The fraction containing mainly proanthocyanidins caused proliferation and activation of ãä T cells, as well as the activation of B cells. The fraction containing mainly anthocyanins slightly activated NK cells detected as an increased expression of NKG2D on NK cell surfaces. The polyphenol fraction increased B cell proliferation, and markedly increased expression of VLA‐1, about 10 fold, on monocytes. VLA‐1 is a cell adhesion molecule. PBMC secreted a number of cytokines in response to incubation with the cranberry fractions; the proanthocyanidin‐containing fraction having the greatest stimulatory effect. These results suggest that cranberry phytochemicals have the potential to immuno‐modulate in a variety of ways. Supported by Ocean Spray Cranberries, Inc and FAES