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Hepatoprotective Effect of Dehydroglyasperin C against Carbon Tetrachloride‐Induced Tissue Damage in Mice Model
Author(s) -
Kim JonSang,
Seo JiYeon,
Kim HyoJung,
Lim JiSun,
Park JiA,
Nam Dae Hwan,
Hong YeSeul,
Han Min Young,
Lim Soon Sung,
Park Jung Han Yoon
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.541.4
Subject(s) - carbon tetrachloride , antioxidant , oxidative stress , chemistry , glutathione reductase , glutathione , pharmacology , ccl4 , in vivo , biochemistry , hepatoprotection , enzyme , catalase , glutathione peroxidase , biology , microbiology and biotechnology , organic chemistry
Our previous study showed that dehydroglyasperin C (DGC) isolated from licorice had antioxidant activity and induced phase 2 detoxifying enzymes in mouse hepatoma cells. This study was performed to investigate whether DGC enhance the expression of phase 2 detoxifying enzymes in vivo system and protect tissues from oxidative stress‐induced damage. Fifty ICR mice were divided into 5 groups, with 10 mice per group. Mice were injected with vehicle (control), carbon tetrachloride (0.6 mg/kg bw), DGC (5 mg/kg bw), or DGC plus carbon tetrachloride (at 2 different timings of DGC injection). After acclimation period of 2 weeks mice were injected with DGC at 15th day, carbon tetrachloride at 16th day and sacrifice at 17th day after beginning of the experiment. Dehydroglyasperine C induced quinone reductase activity in small intestine, large intestine and kidney in mice. Biomarkers for oxidative stress such as FRAP showed that DGC improved antioxidant potential in mice. Compared with mice injected with CCl4 alone, a group of mice simultaneously injected both DGC and CCl4 had reduced lipid droplet in liver, as assessed by histological examination. Also centrilobular liver injury induced by CCl4 injection was alleviated slightly by cotreatment with DGC. In conclusion, DGC appears to have hepatoprotective effect maybe through modulation of antioxidant enzymes such as quinone reductase.