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Arterial tissue culture medium accelerates anthocyanin degradation and polymeric formation in extracts from chokeberry, bilberry and elderberry
Author(s) -
Bell David Robert,
Nalliah Ruth E,
Cressman Scott J,
Swartz Andrew J,
Moon Haley
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.540.7
Subject(s) - anthocyanin , bilberry , chemistry , monomer , degradation (telecommunications) , food science , biochemistry , tissue culture , polymer , chromatography , in vitro , organic chemistry , telecommunications , computer science
We examined whether anthocyanin degradation and polymeric formation in extracts from chokeberry (Ch), bilberry (B) and elderberry (E) occurs in tissue culture media as reported for extracts in salt solutions used for arterial vasoreactivity studies. Extract solutions at 0.8 or 0.08 mg/ml were made in sterile HEPES buffered DMEM and stored at 37°C in or out of an O 2 /CO 2 tissue culture incubator. Samples were measured for monomeric anthocyanin concentration and polymeric color formation over 72 hours. Monomeric anthocyanin concentration in medium diminished over time (Ch = 50%, E = 28%, B = 4% of 0.8 mg/ml remaining at 24 hrs). CO 2 accelerated monomeric degradation in all extracts (Ch = 0%, E = 10%, B = 2% of 0.8 mg/ml remaining at 24 hrs). Degradation of extracts at 0.08 mg/ml was generally greater. In all cases an increase in anthocyanin polymeric formation paralleled the rate of monomer degradation. Thus tissue culture medium transforms monomeric anthocyanins into anthocyanin polymers or addition products at rates dependent on the individual anthocyanin composition and medium conditions. This transformation must be taken into account in interpreting studies that examine effects of anthocyanins in cell culture systems and raises the possibility that anthocyanin polymers may play a role in in vivo biological effects of these extracts. (Support: The Ferne and Audry Hammel Fund of Huntington University, Huntington, IN)