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Improved prep‐scale isolation of glucosinolates from cruciferous vegetables using a novel stationary phase
Author(s) -
Scholl Chris,
Barnes David,
Hanlon Paul
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.540.18
Subject(s) - glucosinolate , chemistry , chromatography , degradation (telecommunications) , cruciferous vegetables , metabolite , myrosinase , biochemistry , biology , botany , computer science , telecommunications , genetics , cancer , brassica
Interest in glucosinolate (GLS) metabolites, such as isothiocyanates (ITCs), calls for a fast and efficient prep scale isolation method for glucosinolates. Raphasatin, the ITC metabolite of glucoraphasatin the most common GLS in radishes, is unstable in an aqueous environment. The degradation product(s) of raphasatin have been shown to induce detoxification enzymes, a proposed mechanism of chemoprevention. Isolation of the quantity of raphasatin required for the chemical identification of its degradation product(s) requires a preparative liquid chromatography (PLC) method that is able to resolve glucoraphasatin from glucoraphenin, a GLS redox pair which are the most abundant GLSs in radishes. Separation of glucosinolates with a standard C18 stationary phase was compared with separation with a novel stationary phase, Agilent Zorbax Bonus‐RP, a C14 alkyl chain with an embedded amide group. The Bonus‐RP significantly improved resolution of glucoraphasatin and glucoraphenin as well as GLSs present in broccoli and other cruciferous vegetables.