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Quercetin causes dose‐related increases in energy expenditure and PGC1α in muscle of C57BL/6J mice
Author(s) -
Henagan Tara Michelle,
Lenard Natalie R,
Rietschier Helena,
Anaya Stephanie,
Gettys Thomas W,
Cefalu William T,
Stewart Laura K
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.540.15
Subject(s) - quercetin , respiratory exchange ratio , medicine , endocrinology , ppargc1a , chemistry , energy expenditure , peroxisome proliferator activated receptor , antioxidant , coactivator , receptor , heart rate , blood pressure , biochemistry , transcription factor , gene
We explored the protective effect of varying doses of the antioxidant quercetin on mitochondrial function in C57BL/6J mice on a high fat (HF) diet. Six week old male C57BL/6J mice (N = 192) were randomly assigned to one of the following diets: HF (45% kcal fat), HF + 600ug quercetin/day (600Q), HF + 250ug quercetin/day (250Q) or HF + 50ug quercetin/day (50Q). Body weight (BW), body composition (BC), and food consumption (FC) were measured weekly. At 3 weeks (wk) and 8 wk, energy expenditure (EE), respiratory exchange ratio (RER), and activity were assessed. At the 3 and 8wk time points, animals were sacrificed and quadriceps muscle was used for quantification of peroxisome proliferator‐activated receptor gamma coactivator‐1 alpha (PGC1α) mRNA by real time PCR. The assessed variables did not differ at 3 wk, but at 8 wk 50Q significantly increased EE (P < 0.05) but did not change PGC1α Conversely, the 600Q group exhibited a decrease in EE yet higher levels of PGC1α in comparison to HF (P < 0.05). 250Q had no difference in EE at 8 wk but increased PGC1α (P < 0.05). There were no differences in BW, BC, or FC among groups at 8wk. We conclude that 8wk of quercetin supplementation causes dose‐related metabolic responses that may be indicative of protective mitochondrial adaptations to a HF diet, independent of a reduction in BW or a change in BC. Grant Funding Source : Botanical Research Pilot Funding Program ‐ Pennington Biomedical Research Center