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Effects of lutein and β‐cryptoxanthin on MMP‐13, PGE2 and cytokine production in human chondrosarcoma cells stimulated with IL‐1α in vitro
Author(s) -
Chew Boon P,
Di Filippo Michael M,
Mathison Bridget
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.539.9
Subject(s) - chondrocyte , chemistry , matrix metalloproteinase , cytokine , in vitro , chondrosarcoma , tumor necrosis factor alpha , microbiology and biotechnology , endocrinology , medicine , biochemistry , biology , pathology
Studies have shown that lutein (Lu) and β‐cryptoxanthin (βCr) may down‐regulate factors involved in inflammation associated with osteo‐ and rheumatoid arthritis. We studied the possible protective effects of Lu and βCr against human chondrocyte dysfunction using the SW‐1353 human chondrosarcoma cell line. Cells were cultured for 24 hr in complete medium containing 0, 0.01, 0.1 or 1.0 μmol/L of Lu or βCr and subsequently stressed with 10 ug/L IL‐1β (final concentration) for 24 hr. Conditioned medium was analyzed for matrix‐metalloproteinase‐13 (MMP‐13), cytokines (IL‐1α, IL‐2, IL‐4, IL‐10, IFN‐γ, IL‐6, IL‐8, and TNF‐α), and PGE2, and the nuclear extract analyzed for NFκB. Lu (1.0 μmol/L; P <0.05) but not βCr decreased MMP‐13. Both Lu (1.0 μmol/L; P <0.05) and βCr (0.1 and 0.01 μmol/L; P<0.01) inhibited PGE 2 production. All concentrations of βCr suppressed (P<0.05) IL‐1α, IL‐2 and IFN‐γ production while Lu increased concentrations of these cytokines. Lu increased (P<0.05) while βCr decreased IL‐4 and IL‐10 concentrations. NFκB p50 production was suppressed (P<0.01) by both Lu and βCr, with Lu being more inhibitory. Therefore, Lu and βCr protected against IL‐1β‐induced chondrocyte dysfunction by down‐regulating NFκB activation and inhibiting inflammatory response, albeit through somewhat different pathways.