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Efficiency of intestinal absorption of beta‐carotene (BC) is not correlated with cholesterol (CHL) absorption in humans
Author(s) -
Fleshman Matthew Kintz,
Cope Keary A,
Novotny Janet A,
Riedl Ken,
Schwartz Steven J,
Jones Peter J,
Baer David J,
Harrison Earl H
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.539.4
Subject(s) - absorption (acoustics) , chemistry , chylomicron , absorption efficiency , zoology , medicine , endocrinology , cholesterol , biochemistry , lipoprotein , biology , materials science , very low density lipoprotein , composite material
The intestinal absorption of BC and of CHL varies among individuals. Experiments in vitro and in mouse models suggested that BC and CHL shared certain molecular mechanisms of absorption. A study was conducted to investigate the variability in BC absorption and its conversion to vitamin A and to compare the efficiency of absorption of BC with that of CHL. Ten men consumed a 5 mg dose of deuterium labeled BC (BCd8), with 6 subjects repeating the dose 2 months later. Blood was collected hourly for 9 h, and enrichment of chylomicrons with BCd8 and retinyl‐d4 esters (REd4) was determined by HPLC‐MS. CHL absorption was measured in the same subjects by the oral/IV dual isotope ratio method using deuterated CHL and 13C CHL and ranged from 28% to 60%. Chylomicron BCd8 AUC ranged from 0.047 to 0.414 μM‐hr and the CM REd4 AUC ranged from 0.102 to 2.59 μM‐hr. For subjects who repeated the treatment, the total BC absorbed at the two times was correlated (r=0.99; p<0.0001). The conversion of BCd8 to REd4 ranged from 37–77%, and the extent of conversion at the two times was correlated (r=0.77; p=0.08). There is marked inter‐individual variability in BC absorption and conversion to RE. In contrast, intra‐individual variability in BC absorption and its conversion to RE is low. Importantly, there was no correlation between an individual's efficiency of absorption of BC and their efficiency of absorption of CHL (r=0.21; p=0.56). HL049879 & USDA/ARS