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Cancer Chemotherapeutic Effects of Modified Sodium Silicate (Alkahydroxy/Alka V6)
Author(s) -
Townsend Deana,
White Lauren,
Chesnut Troy,
Duesler Sandra,
Cisneros Ignacio,
Richardson C. Reed,
Vattem Dhiraj
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.537.16
Subject(s) - chemistry , superoxide dismutase , malondialdehyde , nuclear chemistry , catalase , glutathione , biochemistry , in vivo , pharmacology , antioxidant , enzyme , biology , microbiology and biotechnology
A proprietary modified sodium silicate manufactured by Cisne enterprises Inc. (Odessa, TX) was evaluated for its ability to modulate various parameters relevant to establishment and progression of cancer. Antimutagenic effects were determined using Ames test. Prevention of colon cancer cell (HT‐29) attachment and growth was done using standard methods. Apoptotic induction was measured by DNA fragmentation (DNAF) assay. Malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) activity were measured using standard assays. Chemical structure determined by nuclear magnetic resonance (NMR) and infra‐red (IR) spectroscopy suggested that the product was a mixture of trimeric sodium silicate and sodium silicate pentahydrate. A dose‐dependent reduction in attachment (EC50 = 3450 ppm) and growth (EC50 = 4000ppm) of HT‐29 was observed. At low levels (250–25000 ppm) the product was able prevent various sodium azide induced mutations in Ames test. A dose dependent increase in DNAF suggested induction of apoptosis. A drop in MDA levels and increased in GHS, SOD and CAT activities suggested induction of antioxidant response. We conclude that the product may have cancer chemotherapeutic properties in vitro due to its unique structural and electrochemical properties. In vivo tests are imperative to determine true effectiveness. Funding source: Cisne Enterprises Inc.