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Investigating the Complex Formation between Boron and Riboflavin using Electrospray Ionization Mass Spectrometry and Fluorescence Spectroscopy
Author(s) -
Kim Danny H,
Faull Kym F,
Eckhert Curtis D
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.537.13
Subject(s) - riboflavin , chemistry , electrospray ionization , boron , mass spectrometry , fluorescence , fluorescence spectroscopy , chromatography , biochemistry , organic chemistry , physics , quantum mechanics
Boron, found richly in green vegetables, fruits, peanuts and legumes, has high affinity for cis‐diol functional groups found in nucleotides, sugars, as well as in vitamins such as riboflavin. For the first time, electrospray ionization mass spectrometry in the negative ion mode was utilized to observe the complex formation between boron and riboflavin. In addition, fluorescence spectroscopy was used to investigate whether boron can affect the binding affinity between riboflavin and riboflavin binding protein from chicken egg white. The results of this study can help support the conclusions made from previous work in which a correlation between boron exposure and riboflavin deficiency in chicks and hens was demonstrated. The results of this work are relevant for the treatment for human riboflavin deficiency that causes dry skin, photo‐sensitivity and inflammation of the lining of mouth and tongue.