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Protein Analysis Using Micro‐Electrophoresis and Micro‐Spectroscopy
Author(s) -
Whitson Matthew McGuire,
Mancuso Matthew Cian,
Harwood John J,
Boles Jeffrey Oakley
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.531.8
Subject(s) - graduate students , instrumentation (computer programming) , computer science , chemistry , electrophoresis , chromatography , nanotechnology , materials science , medical education , medicine , operating system
Since the development of the Bradford and Lowry protein assays in 1976 and 1951, respectively, many additional techniques for the determination of protein concentration have been developed based on dye‐binding technologies. In an effort to exploit current available technologies and at the same time expose undergraduate and graduate students to emerging techniques, we carried out a comparative analysis using both traditional methods and methods exploiting micro‐electrophoresis and micro‐spectroscopy. We use a bioanalyzer “lab‐on‐a‐chip” instrument in the analysis, which represents an example of a micro‐total analysis system (u‐TAS). We are developing experiments to help chemistry students in their junior year become more familiar with biotechnology instrumentation that can be used in undergraduate and graduate research. Our poster will present the theoretical principles demonstrated in these teaching experiments, and practical details on how to include both micro‐electrophoresis and micro‐spectroscopy in chemistry and biochemistry courses. This research has been supported by the National Science Foundation.