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Electrochemical and Spectroscopic Studies of Stellacyanin of Japanese Lacquer Tree Sap from Oku‐kuji, Japan
Author(s) -
Takahashi Rika,
Terakado Hideto,
Kohzuma Takamitsu
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.521.7
Subject(s) - chemistry , electrochemistry , electrode , cyclic voltammetry , raman spectroscopy , electron transfer , analytical chemistry (journal) , photochemistry , organic chemistry , physics , optics
Stellacyanin from Japanese lacquer tree is a member of blue copper protein, which shows an intense absorption at ~600 nm. The electrochemical and spectroscopic properties of stellacyanin have been investigated by cyclic voltammetry, electronic absorption, and resonance Raman (RR) spectroscopy. Stellacyanin was extracted and purified from the acetone powder of the lacquer tree sap provided by Oku‐Kuji Laquer Tree Sap Union. Cyclic voltammograms of stellacyanin were recorded at various promoter modified gold electrodes to know the electron transfer manner of the protein. Stellacyanin showed well defined quasi‐reversible electrochemical behavior at 4, 4_f‐pyridinedisulfide (4‐PDS), 3‐dithiopropionate (3‐DTP), or mercaptosuccinate (MSA) modified Au electrode, and the reduction potential of stellacyanin from Oku‐Kuji was estimated to be 161 mV at pH 7.0. The quasi‐reversible behavior of stellacyanin was not observed at a diethylaminoethanethiol (DEAET) at pH 7.0 and 3‐DTP or MSA/Au electrode at pH 11.0. These results could be concluded that the surface conserved lysine residue of stellacyanin is important for the electron transfer. Related spectroscopic studies will be reported. This work was supported by the Project of Development of Basic Technologies for Advanced Production Methods Using Microorganism Functions by the New Energy and Industrial Technology Development Organization (NEDO).