Premium
Endocytosis of pro‐apoptotic twin‐base RGDSK helical rosette nanotubes in human U937 macrophage cell line
Author(s) -
Singh Suri Sarabjeet,
Fenniri Hicham,
Hemraz Usha,
Singh Baljit
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.520.7
Subject(s) - endocytosis , endosome , microbiology and biotechnology , rosette (schizont appearance) , intracellular , nanocarriers , biology , biophysics , chemistry , cell , materials science , nanotechnology , biochemistry , drug delivery , immunology
Twin‐base helical rosette nanotubes (RNTs), a novel class of biologically inspired nanotubes, hold tremendous potential as targeted drug delivery shuttle. To realize biomedical potential of RNT, there is a critical need to understand the interaction of RNT with the cells. Therefore, we synthesized hybrid RNT that composed of RGDSK and FITC to gain ability to track them during in vitro experiments with human differentiated macrophages. Scanning electron microscopy confirmed nano‐scale dimensions of RGDSK RNTs. FITC‐RGDSK RNT was co‐localized with integrin avb3‐Cy5 on the macrophages within 2 minutes of incubation at 37 deg C. Integrin avb3 and flotallin‐1, a lipid rafts protein, showed fever co‐localization in non‐stimulated cells. FITC‐RGDSK RNT also co‐localizes with EEA1, an early endosome marker. The inhibition of the co‐localization of FITC‐RGDSK RNT with the integrin and translocation into endosomes at 4 deg C demonstrated energy‐dependence of the process. The incubation of differentiated macrophages with RGDSK‐TBL RNT (>5 μM) resulted in nearly 100 % apoptosis within 12 hours. These results suggest that the FITC/RGDSK‐TBL RNT could be used as a versatile platform to deliver a variety of biologically active molecules for cancer therapy. Support: NSERC, AARI. Grant Funding Source: AARI, NSERC