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The electrode‐electrolyte impedance spectroscopy measurements of cancer cell culture media and KCl (potassium chloride) while calibrating a system throughout a frequency range
Author(s) -
Richardson Clarice,
Bhansali Shekhar,
Vuppala Geetha
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.503.4
Subject(s) - electrical impedance , electrolyte , electrode , dielectric spectroscopy , resistor , compensation (psychology) , materials science , analytical chemistry (journal) , chloride , electrical engineering , optoelectronics , voltage , chemistry , engineering , electrochemistry , chromatography , psychology , psychoanalysis , metallurgy
The study and application of how systems are calibrated throughout a frequency range and impedance measurements are vital skills. The research objectives were to analyze how to calibrate a system to obtain correct readings throughout a frequency range and record the impedance of standard KCL (Potassium Chloride) and different cell culture media. Two methods that were used to calibrate the system were: 1. Open and short compensation and 2. Open, short and load compensation. We identified a system, Multi Micro‐Electrode Bio‐Sensor, located at Univ. of South Florida's Electrical and Biomedical Engineering Laboratory and conducted tests to study electrode‐electrolyte interface of impedance spectroscopy measurements. To understand how to calibrate the system, we built a circuit using the leads that come from the PG Stat 30 instrument which supplies the voltage and current and placed a load (10.7 KΏ resistor) across the leads. We found at lower frequencies, we obtained the correct value of the load resistor; however, at high frequencies, we obtained incorrect results. We also used KCL (Potassium Chloride) to study and understand the physical aspects of the Biosensor System. The Biosensor is also used to measure Electrode‐Electrolyte Impedance Spectroscopy of Cancer Cell Culture Media and KCL. Future work will focus on the long‐term analysis of the Multi Micro‐Electrode Bio‐Sensor System.