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Indole‐3‐Acetic Acid/Horseradish Peroxidase Induces Apoptosis in TCCSUP Human Urinary Bladder Carcinoma Cells
Author(s) -
Jeong Yun Mi,
Kim Su Yeon,
Li Hailan,
Yun HyeYoung,
Baek Kwang Jin,
Kwon Nyoun Soo,
Kim Won Yong,
Kim DongSeok
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.503.3
Subject(s) - apoptosis , viability assay , programmed cell death , dna fragmentation , horseradish peroxidase , chemistry , poly adp ribose polymerase , microbiology and biotechnology , biology , cancer research , biochemistry , enzyme , polymerase
In the present study, we determined the effects of IAA/HRP treatment on TCCSUP human urinary bladder carcinoma cells. IAA/HRP combination decreased cell viability of TCCSUP cells in a time‐ and dose‐dependent manner, whereas IAA or HRP alone showed no such effect. In addition, the decreased cell viability was restored by pretreatment with ascorbic acid. To clarify the mechanism of death of TCCSUP cells by IAA/HRP, we investigated the signal transduction pathways related to the apoptosis. IAA/HRP activates p38 mitogen‐activated protein (MAP) kinase and c‐Jun N‐terminal kinase (JNK). We further investigated the IAA/HRP‐mediated apoptotic pathways and showed that IAA/HRP induces caspase‐8 and caspase‐9 activation, which results in caspase‐3 activation and poly(ADP‐ribose) polymerase (PARP) cleavage. To further confirm whether IAA/HRP induces apoptotic cell death, we performed a DNA fragmentation assay after IAA/HRP treatment and found that IAA/HRP‐treated cells showed typical apoptotic DNA ladder formation. From these results, we suggest that IAA/HRP induces apoptosis of TCCSUP human urinary bladder carcinoma cells via both death receptor‐mediated and mitochondrial apoptotic pathways.