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Chronic β3 adrenergic stimulation induced remodeling of white adipose tissue in mice
Author(s) -
Lindholm Chris R,
Bauwens Jake D.,
Ertel Rebecca L.,
Mulligan Jake D.,
Schmuck Eric G.,
Saupe Kurt W.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.502.3
Subject(s) - medicine , endocrinology , adipose tissue , white adipose tissue , adiponectin , stimulation , adipocyte , adipokine , leptin , adrenergic , brown adipose tissue , obesity , chemistry , receptor , insulin resistance
Obesity and associated co‐morbidities are major health concerns. Here we define how a potential pharmacological treatment for obesity ( in vivo chronic stimulation of β3‐adrenergic receptors with CL 316,243) affects remodeling of intra‐abdominal fat and specific serum markers closely related to obesity‐associated co‐morbidities. Male C57Bl/6 mice (n=8/group) were studied during 14 days of either no intervention (controls) or CL infusion (1mg/kg/day via Alza pump) with epididymal fat pads collected at sacrifice. CL treatment had no effect on food intake or body weight but decreased epididymal fat pad mass from 250±29 to 121±19 mg (p<0.01). While CL treatment did not change the protein concentration of whole adipose tissue or the non‐adipocyte cell fraction, it increased protein concentration in the adipocyte fraction > 4‐fold (0.2±0.04 to 0.9±0.09 mg/ml, p<0.01). CL treatment decreased blood glucose from 202±10 to 144±10 mg/dl (p<0.01) and serum leptin from 2.0±0.2 to 1.3±0.2 ng/ml (p<0.01). The cardioprotective adipokine adiponectin was increased from 6.2±0.3 to 8.3±0.3 μg/ml (p<0.01) by CL treatment. We conclude that the favorable serum changes induced by β3‐adrenergic stimulation are not dependent on decreased body weight but instead are likely linked to a remodeling of adipose tissue involving primarily the adipocytes.