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Association Between 5‐HT Receptor Gene Promoter Polymorphism and Obesity
Author(s) -
Hemimi Neveen Salah El Din,
El Salam Mona Mohamed Abd,
Khalil Marwa Mahmoud,
Ahmad Heba Mohamed Galal El Din
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.502.1
Subject(s) - genotype , obesity , medicine , allele , endocrinology , promoter , polymorphism (computer science) , gene polymorphism , gene , restriction fragment length polymorphism , biology , genetics , gene expression
Background Serotonin has an integral role in appetite control, sexual and social behaviour, and stress response and these all form part of the individual susceptibility to obesity. AA genotype of 5‐HT 2A gene promoter polymorphism was associated with increase receptor expression and binding. Aim To study the relation between the G1438A polymorphism of the 5‐HT 2A gene and the susceptibility to obesity. Material& Method 69 individuals with BMI < 25 Kg/m 2 and 120 individuals with BMI ≥ 25 Kg/m 2 were enrolled in the study. The G1438A polymorphism of the 5‐HT 2A gene was detected by restriction fragment length polymorphism polymerase chain reaction (RFLP‐PCR) of peripheral blood DNA samples. Analysis of data was done by using SPSS program. Results Genotype frequencies for AA and GA& GG were 25% and 75% respectively in the group with BMI ≥ 25 Kg/m 2 compared to 52.2% and 47.8% in the normal BMI group. Carriers of GA& GG genotype were at a significant high risk of developing obesity when compared with carrier of AA genotype (OR=3.3, 95%CI= 1.7–6.1). Moreover, the mean energy intake per day was significantly increased among carriers of GA&GG genotype when compared to AA genotype (p< 0.05). Conclusion G allele was a risk factor for obesity. Source of Fund: Ain Shams University.