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Nuclear Pore Complex [NPC] and Epigenetic Regulator Proteins Share Conserved Domains for Interaction with Functional Non‐Coding ncRNA
Author(s) -
Wissler Joerg E.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.493.5
Subject(s) - biology , nuclear transport , epigenetics , epigenome , nucleoporin , non coding rna , transcription factor , microbiology and biotechnology , rna , genetics , nuclear protein , dna methylation , cell nucleus , gene , gene expression
OBJECTIVE Functional small hairpin ncRNA may address defined homologous helix‐nucleating domains shared in epigenetic regulator proteins entangled in growth, metabolic syndromes, vascular, cancer, epi‐ and genetic information indexing of the epigenome. Whether they may also participate in nucleocytoplasmic transport was investigated by searching conserved interaction domains in nuclear [epigenome] and NPC proteins [Mol. Biol. Cell 20 Suppl, 534, 2009; Ann. N.Y. Acad. Sci. 1137: 316–342, 2008]. RESULTS Edited, modified, redox‐ and metalloregulated functional small hairpin ncRNA [<200n] as bioaptamers in RNP complexes were obtained from transcriptional RNA output of cells activated by extrinsic [environmental] factors. Several components of chromatin formation modulation and NPC were found having conserved homologous helix‐nucleating structural [proteomic] domains in terms of K / R xxx H [K/R3H], i.e.‐t/s/x K / R /q/n/hxxx H /y/n/q/e/d/r/kx 7‐9 h/xx 7–9 h/xx 5–20 K / R /q/n/e/h‐with accessory canonical basic [R/K]n, R/K‐zipper, SR/K/RS, EF‐hand and/or HxxxH/y/n/q segments. They include nucleosomal assembly, histone deacetylase, DNA‐C5‐methyltransferase, sirtuin, hypoxia‐inducible factor, nuclear pore complex, nucleoporin, importin, exportin and nuclear transport factor protein families. By redox‐ and metalloregulated multivalent interaction with molecular imprints in protein components, such ncRNA may display functions in NPC, its transfer or shuttle factors in synergism with indexing of the nuclear epigenome. CONCLUSIONS The results suggest that in the “terra incognita” of molecular dynamics of NPC and orchestration with genomic and nucleocytoplasmic processes, new functions associated with epigenetic imprinting and inheritance await discovery.