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Salidroside inhibits clinorotation‐induced apoptosis in pulmonary microvascular endothelial cells by activating PI3k/Akt pathway and inactivating of caspase 3
Author(s) -
Kang Chunyan,
Yuan Ming,
Zhou Lin,
Zhang Ye,
Liu Changting
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.485.5
Subject(s) - salidroside , pi3k/akt/mtor pathway , protein kinase b , apoptosis , chemistry , phosphorylation , caspase 3 , rhodiola rosea , tunel assay , microbiology and biotechnology , western blot , biochemistry , biology , programmed cell death , chromatography , gene
We have previously demonstrated that clinorotation induced apoptosis in microvascular endothelial cells, but the underlying mechanisms are still not clear. In this study, we test the hypothesis that clinorotation could induce apoptosis in human pulmonary microvascular endothelial cells (HPMVEC) by inhibiting the PI3K/Akt pathway and activating caspase‐3. TUNEL assay and flow cytometric analysis indicated that 72h clinorotation significantly induced apoptosis in HPMVEC. Western blot results showed that clinorotation inhibited the PI3K protein expression and phosphorylation level of Akt. Caspase‐3 activity assay showed that clinorotation increased the activity of caspase‐3.Salidroside, one of main active ingredients extracted from the root of Rhodiola rosea, inhibited the apoptosis and attenuated the decrease of PI3K protein expression and phosphorylation level of Akt. Furthermore, salidroside also antagonized the activation of caspase‐3. Our results suggest that PI3K/Akt pathway and caspase‐3 involve in the apoptosis of HPMVEC after clinorotation, this effect can be reversed by salidroside, which may have a potential application in astronauts during spaceflight.(Funded by PHRP 06Z048)