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Role of Mst3 in oxidative phosphorylation
Author(s) -
Lin ChiaYing,
Wu HungYi,
Yuan ChiunJye
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.485.18
Subject(s) - microbiology and biotechnology , hela , gene knockdown , cytoplasm , apoptosis , mitochondrion , biology , rna interference , phosphorylation , cell , biochemistry , rna , gene
Mst3 (mammalian Ste20‐like serine/threonine protein kinase 3), a key participant in the stress‐ and drug‐induced apoptosis, was recently shown be present not only in cytoplasm but also in the intermembrane space of mitochondria by co‐immunofluorescent staining, scanning electron microscopic imaging and immuno‐blotting after subcellular fractionation. Similar results were also observed in human trophoblast cell line 3A‐sub‐E cells, suggesting that the mitochondrial localization of Mst3 is a general phenomenon. Interestingly, the selective knockdown of Mst3 by RNA interference (RNAi) resulted in a significant reduction of the protein level of respiratory transport chain complexes I and III. Further studies showed that HeLa stable clone with selective knockdown of Mst3, HeLa(siMst3), exhibited an increased dependence of glucose for ATP synthesis. Compared with controls, the survival of HeLa(siMst3) stable clone was much sensitive to the deprivation of glucose in the cultural medium leading to apoptosis. The results indicateMst3 plays an important role not only in cell apoptosis but also in physiological function in cell alive.