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Effect of Pathway‐Interconnectors in SEB Induced Apoptosos Related Events in Human PBMCs
Author(s) -
Lillge Cayla,
Klein Becka,
Zindel Kristin,
Mendis Chanaka
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.485.17
Subject(s) - peripheral blood mononuclear cell , apoptosis , caspase 8 , inhibitory postsynaptic potential , caspase , gene , caspase 3 , microbiology and biotechnology , immunology , biology , chemistry , programmed cell death , genetics , neuroscience , in vitro
This study follows a molecular route to better understand the apoptosis related events that are key to the patho‐mechanism of Staphylococcal Enterotoxin B (SEB) in human peripheral blood mononumclear cells (PBMC). The pathway interconnector, JNK, was identified and may have crucial inhibitory effects on the unwanted SEB induced apoptosis. Our attempt was to inhibit JNK by SP600125, evaluate those attempts by altering a set of known apoptosis related genes, and analyze expression patterns by conducting RT‐PCR. The studied genes include Caspase 1, Caspase 3, Caspase 6, Caspase 7, Caspase 8, Caspase 9, Caspase 10, Heperanse precursor (HEP), SOD, and USP. Further analysis and confirmation was conducted by carrying out a set of protein expression analysis. We believe JNK has the ability to sustain longer lasting inhibitory effects and that it may benefit other experimental models that focus on disease prevention and diagnostics. Dr. Marti Jett ‐ ASBMB