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Kinetics of yeast dissociation from lectin beads: II. alpha methyl glucose
Author(s) -
Zem Gregory C.,
Dreyfuss J,
Allen J,
Kawashima R,
Daco E,
Kanda Y,
Yaghoobian J,
Cochrane A,
Sobhani J,
Pouromid K,
Jahanian S,
Solati A,
Balazadeh H,
Hernandez D,
Cortes M,
Kermani K,
Oppenheimer S. B.
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.480.14
Subject(s) - yeast , concanavalin a , chemistry , sugar , mannose , chromatography , lectin , elution , kinetics , reagent , biochemistry , in vitro , organic chemistry , physics , quantum mechanics
As concanavalin A (Con A) has preferential binding specificity for mannose and glucose, here we test the ability of alpha methyl glucose to dissociate yeast ( Saccharomyce s cerevisiae) from Con A beads. We chose only one concentration of each of these two sugars (mannose sugar in Part I of this series) to study so that we could do hundreds of replicate trials to statistically evaluate the reliability of this new model system. Future dose‐response experiments will be performed to precisely determine the effectiveness of the reagents at the lowest concentrations. In 247 repeated trials using 1.5 M alpha methyl glucose versus controls in the absence of sugar, with tiny Standard Errors, the sugar substantially increased dissociation of yeast that were bound to Con A derivatized beads over the 60 min time course compared with sugar‐free controls (2‐sample‐t‐test results: 20 min p less than 0.0001; 40 min p less than 0.0001; 60 min p less than 0.0001). Repeated measures ANOVA indicated a significant effect of the treatment on the number of bound yeast (F= 333, p less than 0.0001), as well as a significant interaction between treatment and amount of time elapsed (F=152, p less than 0.0001). The results are expected but the model is new. We believe that for the first time the kinetics of yeast elution from lectin beads is precisely measured in an easily visualized, simple and inexpensive model system to test elution reagents in glycan purification protocols and to test the efficacy of potential carbohydrate‐based drugs in dissociating pathogens from human cells (Supported by NIH NIGMS SCORE S0648680, MARC, RISE, the Joseph Drown Foundation and the Sidney Stern Memorial Trust).