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Effects of Omega‐3 Fatty Acids on Lipid Metabolism and Signaling Using Lipidomic Analyses
Author(s) -
Brooks Joshua D,
Norris Paul C,
Dennis Edward A
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.475.1
Subject(s) - docosahexaenoic acid , eicosapentaenoic acid , lipidome , arachidonic acid , lipidomics , eicosanoid , fatty acid , biochemistry , lipid metabolism , lipid signaling , chemistry , metabolism , omega 3 fatty acid , cyp2c8 , fatty acid metabolism , polyunsaturated fatty acid , eicosanoid metabolism , enzyme , cytochrome p450 , cyp3a4
While arachidonic acid (an omega‐6 fatty acid) and its metabolites play a role in many inflammatory diseases, the omega‐3 fatty acids eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) possess potent anti‐inflammatory properties and exert therapeutic benefits. Increased omega‐3 fatty acid intake has been shown to alter the balance of pro‐inflammatory omega‐6‐derived eicosanoid production, but research to date has focused on only a few select lipid metabolites rather than on changes in the cellular‐wide eicosadome. Herein we report overall effects of EPA and DHA on both the cellular lipidome and eicosadome occurring in RAW264.7 macrophages using novel mass spectrometry techniques (JBC, 282 , 22834). Our studies have demonstrated that while small variations are seen in the quantity of omega‐6 fatty acids found in the cell, major decreases are seen in some (but not all) of the known omega‐6 fatty acid‐derived eicosanoids forming in macrophages along with a concomitant increase in a few specific omega‐3 fatty acid metabolites. Thus, for the first time, the overall variation in cellular lipid metabolites caused by the presence of EPA and DHA has been examined. Taken together, these studies will help to elucidate the likely signaling molecules responsible for and the molecular mechanisms underlying the beneficial effects of EPA and DHA in inflammation. This work was supported by NIH Grant GM069338.