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A Proteomic Investigation of Arsenic and Antimony Stress on Sulfolobus solfataricus
Author(s) -
Rastedt Danielle Elizabeth,
Grypiuk Nicole,
Selvig Kyla,
Maaty Walid,
Bothner Brian
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.467.7
Subject(s) - sulfolobus solfataricus , antimony , arsenic , archaea , sulfolobus , proteome , proteomics , chemistry , biology , biochemistry , inorganic chemistry , organic chemistry , gene
Members of Archaea, the third domain of life, have adapted to some of the most severe environments known to support life. One in particular, Sulfolobus solfataricus, is thermoacidophilic, therefore, it can be found in extreme environments such as the hot springs in Yellowstone National Park. This archeaon can endure extreme temperatures, high acidity, and highly enriched metal environments. Understanding how these organisms survive and what particular mechanisms are used in these harsh environments have become of intrinsic interest. The aim of this study was to quantitatively define the regulation of proteins upon treatment with arsenic and antimony species in different time periods and concentrations. Proteomic analyses using two‐dimensional differential gel electrophoresis (2D‐DIGE) was used to determine proteins of interest after exposure to 50μM antimony. Furthermore, SDS‐PAGE analysis was used to determine insoluble proteins after being stressed with 240μM and 1mM arsenic. Regulated proteins lists were created, which can be further used to identify the mechanisms of proteomic response. Together these experiments begin to identify important proteins, which play a role in the environmental stresses of arsenic and antimony.