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Discovering Interactions Present During the Growth and Development of a Craniofacial Bone
Author(s) -
Duench Kellie Lynn,
FranzOdendaal Tamara
Publication year - 2010
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.24.1_supplement.452.4
Subject(s) - intramembranous ossification , endochondral ossification , anatomy , biology , patched , mesenchyme , calvaria , ossicles , cartilage , craniofacial , bone morphogenetic protein , microbiology and biotechnology , ossicle , hedgehog , middle ear , genetics , signal transduction , gene , embryo , in vitro
The study of skeletal (bone and cartilage) development includes the investigation of intramembranous bones of the head and facial skeleton as well as endochondral bones (e.g. long bones) of the body. The development of skeletal tissues often relies on an interaction between the mesenchyme and the epithelium. Bone is formed by signalling interactions between these two tissues. Often these signals experience cross‐talk, where one signal is inducing or inhibiting another signal. When it comes to scleral ossicles (an example of intramembranous bone), it has recently been shown by our lab that the Hedgehog gene family is involved in its development. Using whole mount in situ hybridization further investigation was performed to discover if multiple members of the Hedgehog family are involved, and also to determine the location and distribution of the Patched (Ptc) receptor. Bead implantations using a common Bone morphogenetic protein inhibitor, noggin, were performed to determine the involvement of the BMPs during ossicle development. The relationship and interactions between these two genes families ( BMP , and Hedgehog ) during scleral ossicle development will be discussed. These investigations have allowed for further understanding of the mechanism of development for the ocular skeleton and intramembranous bones, in general. Grant Funding Source: NSERC